Frequently Asked Questions
Clones can be ordered via
here
pBluescript II KS+ (Stratagene pBluescript info page)
- The Adult Cerebrum, Adult Cerebellum and Adult Brain Other Parts tissues were from the White Leghorn IAH strain of RPL Line 15I.
- All embryonic tissues - White Leghorn 'Hisex' breed.
- Adipose - Mixture of Ross 308 broilers + Lohman Brown Hybrid layers (Male and Female)
- Chondrocyte - Misture of Ross 308 broilers (Male and Female)
- Muscle - Mixture of Ross 308 broilers + Isa Brown layers (Male and Female)
- Ovary - Bantam (Layer, Female)
- Pancreas - Mixture of Ross 308 broilers + Isa Brown layers (Male and Female)
Note: IAH = Institute of Animal Health, RPL = Regional Poultry Laboratory, East Lansing, Michigan
On the main page, click on the link to www.chick.manchester.ac.uk and
read the messages for further instructions (the data is in pub/chickest/fastafiles/).
Data can be downloaded for each of the individual sequencing projects or
individual tissues.
At present the trace files for individual EST reads can be downloaded from the information page for that EST
(e.g. 603103685F1).
These trace files represent the un-clipped (raw) EST sequences. Quality scores for the clipped EST sequences will be made available soon (via. the EST information page).
We are currently working on an in-silico subtraction technique to isolate clones which are potentially unique to any given tissue or set of tissues.
This will be available via this site in the near future.
If there are any subtractions you wish to carry out in the meantime, please get in touch with
Paul.E.Boardman@umist.ac.uk who is working on this system and has provided custom subtractions for
a number of researchers to date.
Please view the following page for a more complete discussion on this topic.
Genomic contamination FAQ
Please view the following page for a more complete discussion on this topic.
Hit rate FAQ